Effect of Isolated Bioactive Compounds from Erythroselinum atropurpureum against Biofilm Forming Pathogens

Abstract

Biofilm is the community of microbial cells that attach to living or non-living things. Biofilm members communicate and exchange experiences, and virulence factors with each other, which is called quorum sensing. The main objective of this study was to evaluate the effect of isolated bioactive compounds from Erythroselinum atropurpureum against pathogens microbes. The bark of Erythroselinum atropurpureum was extracted using hexane, ethyl acetate, and methanol for 72h and soaked at room temperature. Antibiofilm was tested by test tube, swarming motility, and inhibition of violet colour production test methods. The results indicate that ethyl acetate extract of Erythroselinum atropurpureum and its purified compounds exhibited the strongest antimicrobials, antibiofilm activity. The antimicrobials activity of the ethyl acetate extract against S. aureus and C. albicans ranged between 5.5-22.7mm inhibition zones. The test tube methods indicate that 2.5mg/mL of extract were inhibited the biofilm formation of S. aureus. Anti-swarming activities were shown by 2.5mg/ml of the extract on P. aeruginosa, S. aureus and the highest inhibition of violacein production of S. aureus, P. aeruginosa, and C. albicans 78.6%, 69.7%, and 67.3% respectively.  The Erythroselinum atropurpureum examined in this study has been varying degrees of antimicrobial activity against bacterial and biofilm forms with some having good activity against both forms. This selected medicinal plant might be potential candidates for further investigation to isolate, synthesis antimicrobial compounds and determine the mechanism of activity.