In Vitro Regeneration of Sweet Potato (Ipomoea Batatas (L.) Lam.) Convolvulaceae, From Leaf and Petiole Explants

Abstract

Biotic stresses are the major constraints that result in total loss
of sweet potato (Ipomoea batatas (L.) Lam.) yield. The establishment of in
vitro regeneration protocol for this plant is essential to improve it through
tissue culture and genetic engineering. The objective of this study was to
establish in vitro regeneration protocol for sweet potato varieties Beletech
and Awassa-83 using leaf and petiole explants. MS medium containing
different concentrations of 2,4-D and kinetin were used to evaluate their
effect on callus induction whereas different concentrations of BAP were used
to evaluate its effect on shoot regeneration from callus. BAP alone or in
combination with GA3 was used to evaluate their effect on shoot
multiplication whereas IBA was evaluated for its effect on rooting. The
highest percentage of callus induction (93%) was obtained from petiole
explants of both varieties on MS medium containing 0.05 mg/l 2,4-D in
combination with 0.5 mg/l kinetin. The highest percentage of shoot
regeneration from leaf explants was obtained on growth regulators-free MS
medium while the best regeneration from petiole explants was obtained on
MS medium supplemented with 1.0 mg/l BAP in both varieties. The highest
number of shoots per node (2.40 ± 0.11) was obtained on MS medium
containing 1.0 mg/l BAP from shoots originally obtained from callus of leaf
explants of Awassa-83. All shoots cultured on MS media containing different
concentrations of IBA and the growth regulators-free medium resulted in
100% rooting. After one month of acclimatization, more than 90% of
plantlets survived